| antibody | Antibody Description | Target | Target Description | Vendor ID | Lab | Documents | Lots | Target Link | Label | |
|---|---|---|---|---|---|---|---|---|---|---|
| CTCF | Rabbit polyclonal. Antibody Target: CTCF | CTCF | CTCF zinc finger transcription factor. A sequence specific DNA binding protein that functions as an insulator, blocking enhancer activity. It has also been suggested to block the spreading of chromatin structure in certain instances. | Millipore 07-729 | Myers, Hardison, Snyder | Human - CTCF (07-729)(Western blot,Motif Enrichment)
Human - CTCF (07-729)(Western blot) Mouse - CTCF(Western blot) |
1350637 DAM1472197 | GeneCard:CTCF | CTCF (07-729) | |
| H3K27ac | Rabbit polyclonal. Antibody Target: H3K27ac | H3K27ac | Histone H3 (acetyl K27). As with H3K9ac, associated with transcriptional initiation and open chromatin structure. It remains unknown whether acetylation has can have different consequences depending on the specific lysine residue targeted. In general, though, there appears to be high redundancy. Histone acetylation is notable for susceptibility to small molecules and drugs that target histone deacetylases. | abcam ab4729 | Bernstein | Human - H3K27ac(Dot blot) | 31456 | GeneCard:HIST2H3C | H3K27ac (ab4729) | |
| H3K27me3 | Rabbit polyclonal. Antibody Target: H3K27me3 | H3K27me3 | Histone H3 (tri-methyl K27). Marks promoters that are silenced by Polycomb proteins in a given lineage; large domains are found at inactive developmental loci. | Millipore 07-449 | Bernstein, Hardison | Human - H3K27me3(Dot blot)
Mouse - H3K27me3 (07-449)(Dot blot) |
DAM1387952 | GeneCard:HIST2H3C | H3K27me3 (07-449) | |
| H3K36me3 | Rabbit polyclonal. Antibody Target: H3K36me3 | H3K36me3 | Specific for histone H3 tri methylated at lysine 36, weakly reacts with H3K36me2. Marks regions of RNAPII elongation, including coding and non-coding transcripts. | abcam ab9050 | Bernstein, Ren, Hardison | Human - H3K36me3(ab9050)(Dot blot)
Mouse - H3K36me3 (ab9050)(Dot blot,Western blot) Mouse - H3K36me3 (ab9050)(Dot blot) |
136353 707946 499302 947454 826245 | GeneCard:HIST2H3C | H3K36me3 (ab9050) | |
| H3K4me1 | Rabbit polyclonal affinity purified. Antibody Target: H3K4me1 | H3K4me1 | Histone H3 (mono methyl K4). Is associated with enhancers, and downstream of transcription starts. | abcam ab8895 | Bernstein, Ren, Hardison | Human - H3K4me1(ab8895)(Dot blot)
Mouse - H3K4me1(ab8895)(Dot blot,Western blot) Mouse - H3K4me1(ab8895)(Dot blot) |
383111 720417 | GeneCard:HIST2H3C | H3K4me1 (ab8895) | |
| H3K4me2 | Rabbit polyclonal. Antibody Target: H3K4me2 | H3K4me2 | Histone H3 (di methyl K4). Marks promoters and enhancers. Most CpG islands are marked by H3K4me2 in primary cells. May be associated also with poised promoters. | abcam ab7766 | Bernstein | Human - H3K4me2(Dot blot) | 56293 | GeneCard:HIST2H3C | H3K4me2 (ab7766) | |
| H3K4me3 | Rabbit polyclonal. Antibody Target: H3K4me3 | H3K4me3 | Histone H3 (tri methyl K4). Marks promoters that are active or poised to be activated. | 1)abcam ab8580 2)Millipore 07-473 |
Bernstein, Hardison | Human - H3K4me3(Dot blot)
Mouse - H3K4me3 (ab8580)(Dot blot) Mouse - H3K4me3 (07-473)(Dot blot) |
498616 331024(ab8580) R0903B0054(07-473) | GeneCard:HIST2H3C | H3K4me3 (07-473) | |
| H3K9ac | Rabbit polyclonal A/G affinity purified. Antibody Target: H3K9ac | H3K9ac | Histone H3 (acetyl K9). As with H3K27ac, associated with transcriptional initiation and open chromatin structure. It remains unknown whether acetylation can have different consequences depending on the specific lysine residue targeted. In general, though, there appears to be high redundancy. Histone acetylation is notable for susceptibility to small molecules and drugs that target histone deacetylases. | abcam ab4441 | Bernstein | Human - H3K9ac (ab4441)(Western blot,ChIP-seq) | 455103 550799 | GeneCard:HIST2H3C | H3K9ac (ab4441) | |
| H4K20me1 | Rabbit polyclonal. Antibody Target: H4K20me1 | H4K20me1 | Histone H4 (mono-methyl K20). Is associated with active and accessible regions. In mammals, PR-Set7 specifically catalyzes H4K20 monomethylation. NOTE CONTRAST to H3K20me3 which is associated with heterochromatin and DNA repair. | abcam ab9051 | Bernstein | Human - H4K20me1(ab9051)(Dot blot) | 104513 519198 | GeneCard:HIST2H4A | H4K20me1 (ab9051) | |
| Pol2 | Mouse monoclonal, clone 8WG16. Antibody Target: POLR2A | POLR2A | RNA Polymerase II | Covance MMS-126R | Farnham, Iyer, Snyder | Human - Pol2(Immunoprecipitation,IP-MassSpec)
Mouse - Pol2(Immunoprecipitation,IP-MassSpec) |
14861301 14922701 E11BF0034 | GeneCard:POLR2A | POL2 (MMS-126R) | |
| Input | Control signal which may be subtracted from experimental raw signal before peaks are called. | Input Control | ||||||||